Monday 6/28: CFU plates were counted.  Results are given below:
 

PASTE EXCEL PICTURE HERE!!!!!!!!!!!!!

Tuesday: Not in lab, moving to Brighton!!

Wednesday: Analyzing data
Thursday: Results obtained in data, Looked for new experiments to start.  T2i cell line needs to be passed (culture needs to be cut down).
            1)Trypsin to cover the monolayer.  Incubate for 2-4 minutes.
            2) Wash 2X with PBS.  Resuspend and count.
            3)20,000 cells in 5-6 mls medium.
Friday 7-2-99:  T2i experiments are planned.  Went to Go-Shau's lab to further analyze experiment 4 data.  New experiment with T2i cell outline:

1)    harvest T2i cells, resuspend in DMEM+FCS
2)    count and adjust to 20,000/ml and put 2 ml/plate onto 35 ml dishes.  Also plate 13000 cells/well for 2 96 well plates.
3) July 5 collect conditioned media from 6 35 mm plates.
4) July 8  collect condited media from all 6 plates.

Experiments comparing the effects of this media to actual Type 2 cells will be conducted next week.